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Reverse Transcription PCR (RT-PCR), is a type of PCR technique that enzymatically amplifies the RNA in vitro. It is the only type of PCR that can amplify the RNA. It uses a reverse transcriptase enzyme in addition to the other basic components of the PCR.

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

Discover the advantages of RT-PCR procedures and see what best fits your needs. See the differences and conditions for two-step and one-step RT-PCR.

The real-time reverse transcription polymerase chain reaction (RT-PCR) uses fluorescent reporter molecules to monitor the production of amplification products during each cycle of the PCR...

完成cDNA合成後，取第一鏈反應液（2–4 μL）以1:5至1:10比例進行PCR擴增。 註： 使用Ready Script ® 試劑時，可直接將2–4 μL未稀釋cDNA加入PCR反應，不會產生抑制作用。 若需儲存，可將cDNA產物以10 mM Tris-HCl緩衝液（pH 8.0）、0.1 mM EDTA稀釋後，於–20 °C保存。

RT-qPCR 可用於多種應用，包括基因表達分析與RNAi 驗證等。 立即點擊鏈接，讓 Thermo Fisher 帶你深入探討定量反轉錄 PCR 的步驟與基本原理。

逆轉錄-聚合酶鏈反應 (Reverse Transcription-Polymerase Chain Reaction，RT-PCR)的原理是：提取組織或細胞中的總RNA，以其中的mRNA作為模板，採用Oligo（dT）或隨機引子利用逆轉錄酶反轉錄成cDNA。